Direct Antiglobulin Test (DAT)

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Direct antiglobulin test (DAT) looks for antibodies attached to red blood cells (RBCs) circulating in the bloodstream. The test may help to detect or identify conditions in which antibodies become attached to RBCs, causing them to break apart (hemolyze).

A positive DAT means that there are antibodies attached to the RBCs.
In general, the stronger the DAT reaction (the more positive the test), the greater the amount of antibody bound to the RBCs.

A negative DAT means that antibodies are most likely not attached to RBCs and the signs and symptoms are due to another cause that requires further investigation.

Keywords: DAT; Direct Coombs Test; Direct Anti-Human Globulin Test; Direct Antiglobulin Test.

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